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KMID : 0370220140580050337
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Yakhak Hoeji
2014 Volume.58 No. 5 p.337 ~ p.342
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The Effect of Dioscorea villosa Derived-diosgenin on Vascular Contractility
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Je Hyun-Dong
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Abstract
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The present study was undertaken to investigate the influence of diosgenin on vascular smooth muscle contractility and to determine the mechanism involved. We hypothesized that diosgenin, the primary ingredient of Dioscorea villosa, plays a role in vascular relaxation through inhibition of Rho-kinase in rat aortae. Denuded arterial rings from male Sprague-Dawley rats were used and isometric tensions were recorded using a computerized data acquisition system. Interestingly, diosgenin inhibited fluoride-induced contraction but didn’t inhibit phorbol ester-induced contraction suggesting that additional pathways different from endothelial nitric oxide synthesis such as inhibition of Rho-kinase might be involved in
the vasorelaxation. Furthermore, diosgenin didn’t inhibit thromboxane A2-induced increases in pERK1/2 levels suggesting
the mechanism excluding inhibition of thromboxane A2-induced increases in ERK1/2 phosphorylation. This study provides
evidence that diosgenin induces vascular relaxation through inhibition of Rho-kinase in rat aortae.
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KEYWORD
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diosgenin, endothelium, ERK1/2, fluoride, phorbol ester, Rho-kinase, vasoconstriction
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